Artificial Insemination article by Dr. Talha Javed


                             Artificial Insemination History

·       This procedure was adopted in 1322 A.D.
·       Arabian started this procedure for the first time.
·       They invented this procedure.
·       Liquid semen stored at 4C up to 48 hours at the room temperature.
·       Frozen semen stored in packing in the straw (fence size is .5 million) preserve in a container having -196C (liquid nitrogen) maintained temperature.
Two categories of straws:
Sex semen
Non-sex semen
Sex semen: Both male and female semen.
90% are female for dairy purpose.
10% are for meat purpose.
Non-sex semen:
50% chance of male
50%chances of females
Benefits of artificial insemination:
·        Cheapest and fastest method of genetics.
·        For 25 female cow you need only one bull to serve all 25 females.
·        Cost effective method.
·        Maximum utilization of superior germplasm.
·        Prevents to spread vernal diseases.
·        Prevents the infections of genetic diseases.
·        Long term storage.
·        In 1780 Spallanzani he was the inventor of artificial insemination in bitch
·        In 1899 Ivanoff Russian scientist he performed the procedure of artificial insemination in birds.
·        In 1931 19,800 cows were breed artificially inseminated in Russia.
·        In 1936 AI cooperate society develops
·        In 1938 cooperate society association develops in USA with the name of New Jersey State College
·        In 1949 the improve methods of artificial insemination freezing methods develops.




 Artificial Insemination


Definition of artificial insemination:  Veterinary procedure of injecting semen into the vagina or uterus.
Limitations:
       I.            Require specialist for rectal palpation
     II.            Estrus detection (heat or not in heat)
  III.            Hygiene and sterilization
Techniques of artificial insemination:
1.     Cervical insemination (sheep and goat).
2.     Vaginal insemination (mares).
3.     Recto vaginal insemination (cow and buffalo).
1.     Vaginal insemination: It is an ancient and oldest method of artificial insemination. Semen is deposited through the catheters or tube. (Tube length is 16 inches). The rod should be upward at 45 to 30 angle.  Than the sperm is injected into the sub urethral diverticulum.
Drawback:
        i.            Large amount of volume.
      ii.            Conception chances are going to be low if artificial insemination is performed.
    iii.            Highly motile sperms with good concentration.
2.     Cervical insemination: In this method, spectrum is placed in the vagina of the cow, which provides passage outside to the site of insemination, then inseminating tube is passed through the speculum and semen is deposited at the cervix.
Disadvantages:
Repeated sterilization of the equipment
Low conception rate as compared to the recto vaginal method.
Benefits: this method can be used in the sheep and goats where recto vaginal or other method could not be done.
3.     Recto vaginal insemination:  Remove the waste material first.
Push cranial sight fold going to be in straights position and rod pass through it.
Rod pass through the cervix rings of the cervix.
The method is mostly performed in the large animals by the expert technicians.
Advantage: High conception rate.
Didn’t require any sterilization

Semen collection device:  Semen is collected into a pre warmed insulated or jacketed tube through a funnel or cone. All surfaces coming into contact with semen should be clean, warm, dry and free of spermatoxic agents. Because "cold shock" causes irreversible damage to spermatozoa, efforts to maintain semen at 30-35°C until the "on-site" evaluation procedures are complete is an important consideration for successful semen assessment.

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