Reproduction Division, BRI Pattoki

The reproduction division is established in BRI, Pattoki. It has an Organizational Structure (Click here!). It has a major contribution to development and research. In this report, we have mentioned some important aspects of this division.

Objectives of Reproduction Division:

1.      Undertake research trials to examine the applicability of In Vitro Maturation (IVM) and In Vitro Fertilization (IFV) in buffalo.

2.      To establish a protocol for superovulation, embryo recovery and synchronization of oestrus in buffalo.

3.      Develop an optimal technique for buffalo semen cryopreservation to enhance their overall fertility.

4.      Undertake a study to improve A.I technique and semen handling to obtain a higher reproductive rate.

Procedure for Embryo Transfer:

1.      Take the ovary from the slaughterhouse.

2.      Aspirate ova in ovaries.

3.      Take out the ova from it.

4.      Use the needle of 17-18 gage to aspirate (suck).

5.      Fluid is placed in a test tube.

6.      Take semen from a bull and cross it with the preserved ovum to start the fertilization.

\     Cleavage is formed (oocytes).

7.      It is a seven-day process then we shall place it in the animal or freeze it.

8.      It will be placed with the aid of the fallopian tube and then the buffalo will take ten months for parturition.

In-Vitro Buffalo Embryo Production

Steps of (I.V.F):

In Vitro Fertilization is performed in the lab and is also called as test tube baby technique. In this technique, we aspirate ova from ovaries and mature them in laboratories. 

Steps of In Vitro Fertilization

• 1.      We keep ova in maturation media for 24 hours.
• 2.      Then it will be placed in the fertilization media for 18 hours.
• 3.      In fertilization media, semen or sperms will be crossed.
• 4.      After 18 hours it shall be placed in culture media for seven days.

In Vivo Fertilization:

1.      It is performed in the body of a live animal.

       2.      Artificial insemination is practised with semen from the body of a bull.

Procedure:

1.      Pre-mature ova is taken which will become mature after 24 hours in maturation media.

·         Cell broken twice is called cleavage.

·         A Cell is broken four times (16-32 cells).

·         64 cells are called blastocysts.

2.      Buffalo has 3 follicular waves whereas cow has 2 follicular waves.

3.      In oestrus two to three waves appear.

Outcomes of this Project:

1.      We can take seventy to eighty offspring from a single animal.

1.      More offspring can be taken from a buffalo whereas in a routine it undergoes calving five to six times.

2.      It is easy as the buffaloes are inseminated at the same time which is a time-based synchronization.

3.      It is economically profitable.

Heat Signs:

Some heat signs of buffaloes are given as:

1.      Animal makes noises (bellowing).

2.      Mucus discharge is observed.

3.      The colour of secretion is crystal clear.

4.      The secretion material should hand to joints.

5.      Swelling of vulva occurs.

6.      If the lips of the vulva are opened it shows a red vestibule.

7.      It allows other animals to jump over it.

Test Tube Babies of Buffaloes

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